Tutorials

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Protocols, Application Notes and FAQs relating to fluorescence staining, imaging and image processing.

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Indiana Center for Biological Microscopy

Indiana University School of Medicine

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Now showing 1 - 9 of 9

ICBM-Spinning Disc Microscope System User Notes
(2009-06-17)
Incomplete draft manual for ICBM's Spinning Disc Microscope System (Perkin Elmer) R2-E2.
Zeiss Confocal Microscope User Manual
(2009)
Manual for ICBM's Zeiss LSM-510-UV Confocal Microscope.
Olympus2 User Notes
(2009-07-15)
Manual for ICBM's Olympus-Fluoview1000-MPE Software version 1.7b, R2-E233.
Widefield-Nikon Eclipse Microscope User Manual
(2009)
Manual for ICBM's Widefield-NikonEclipseTE200-ORCA Nikon Eclipse TE200 Inverted Microscope with Hamamatsu 1394 Orca-ER Cooled CCD Camera and Micromanager Software.
BioRad Microscope User Manual
(2009)
Step by step user manual for the ICBM's BioRad MRC 1024 MP Confocal & Multi-Photon Microscope.
FRET
(2009)
Imaging of Fixed Cells
(2009)
Imaging Live Cells
(2009)
This guide is intended to help even novice users to be successful with live cell imaging and generate quality data at the ICBM.
Analysis of Colocalization Using Metamorph
(2009)
Two methods of Colocalization analysis available as plugins in Metamorph are described in this handout: Correlation Plot and Measure Colocalization.